تثبیت ماسه‌های شرق اصفهان به کمک فناوری زیستی باکتری Halomonas sp. در مقایسه با عملکرد باکتری شاهد Sporosarcina pasteurii (SP)

نوع مقاله : مقاله پژوهشی (کاربردی)

نویسندگان

1 استادیار، گروه مهندسی عمران، دانشگاه فنی و حرفه‌ای، تهران، ایران.

2 استاد، گروه مهندسی عمران، دانشگاه VIT، ولور، هند.

چکیده

اخیراً بهبود و مقاوم‌سازی خاک به کمک رسوب‌زایی کربنات توسط میکروب‌ها (MICP) حلقه‌ای بین فناوری زیستی و مهندسی زمین‌شناسی ایجاد کرده است. تکوین چنین روشی می‌تواند هم‌زمان تأثیرات دوسویه مفید زیست‌محیطی و ژئوتکنیکی ایجاد کند. در این پژوهش برای مقاوم‌سازی ماسه‌های شرق اصفهان، از باکتری‌هایHalomonas sp.  و Sporosarcina pasteurii (SP) (استاندارد) استفاده شده است. در این راستا طی دوره‌های 42 روزه آزمایش‌های مختلفی به‌منظور بررسی بهبود مقاومتی خاک انجام گردیده است. در این مطالعه مشخص گردید که خواص مهندسی خاک‌های موردآزمایش با تحریک باکتری‌های موجود در آن به کلسیت‌زایی، به‌صورت قابل‌توجهی تغییر یافته است. بررسی این ویژگی‌ها به کمک آزمایش‌های برش مستقیم و امواج فشاری نشان می‌دهد که مقاومت خاک به کمک باکتری Halomonas sp در مقایسه با باکتری استاندارد (SP) به‌صورت امیدوارکننده‌ای بهبود یافته است؛ به‌طوری که سرعت امواج فشاری در نمونه مقاوم شده توسط باکتری به حدود 390 متر بر ثانیه رسیده است و زاویه اصطکاک داخلی خاک نسبت به باکتری استاندارد با شیب ملایم‌تری افزایش یافته است.  

کلیدواژه‌ها


عنوان مقاله [English]

Sand Soil Stabilization Using Halomonas Bacterial Calcite Precipitation in East of Isfahan and Comparison with Sporosarcina Pasteurii (SP) Bacteria Function

نویسندگان [English]

  • Hoseinali Bagi 1
  • Ali Rahimi 2
1 Assistant Professor, Department of Civil Engineering, Technical and Vocational University, Tehran, Iran.
2 Professor, Department of Civil Engineering, VIT University, Vellore, India.
چکیده [English]

Biocementation is a recently developed link in geological engineering and biotechnology which deals with the application of microbiological activities to improve the engineering properties of soils and creates the positive role of geotechnical engineering for protection of the environment. In this investigation, Halomonas sp. and Sporosarcina Pasteurii (SP) was used for biocementation of sands in eastern area of Isfahan, Iran.  As compared with conventional microbial induced calcite precipitation (MICP) methods, this strategy which uses free urease catalysts to secure bacterial enzyme induced calcite precipitation (MICP) appears to offer an improved means of bio-stabilizing sand soils in a 42day period. After biocementation, the velocity of ultrasonic waves reached approximately 390m/s and the internal angle of friction in bacterial sandy samples increased. Tests were conducted to evaluate the feasibility of using ultrasonic testing for stabilization applications. The ultrasonic testing consisted of determining primary-wave (P-wave) velocities of stabilized mixtures.

کلیدواژه‌ها [English]

  • Stabilization
  • Sands
  • Halomonas bacteria
  • Biotechnology
[1] Verma, R. K., Chaurasia, L., Bisht, V., & Thakur, M. (2015). Bio-mineralization and bacterial carbonate precipitation in mortar and concrete. Bioscience and Bioengineering, 1(1), 5-11. http://files.aiscience.org/journal/article/pdf/70010018.pdf
[2] Karol, R. (2003). Chemical Grouting And Soil Stabilization, Revised And Expanded (3 ed.). CRC Press https://doi.org/10.1201/9780203911815
[3] Sharifi Asadi, D., Ardakani, A., & Garoosi, G. (2018). Investigating effective factors of biocementation soil improvement on sandy soil with different Fine-content. Modares Civil Engineering, 18(2), 127-138. https://mcej.modares.ac.ir/article-16-16953-fa.html
[4] Bagi, H., Atesampour, A., & Rahimi, A. (2021). Environmental significance of benthic foraminifera and microfacies of central Tethyan Upper Triassic strata, central Iran. STRATIGRAPHY, 18(2), 89-102. https://www.micropress.org/microaccess/stratigr aphy/issue-369/article-2230
[5] Mohammadizadeh, M., Ajalloeian, R., Nadi, B., & Nezhad, S. S. (2020). Experimental study on soil improvement using local microorganisms. Arabian Journal of Geosciences, 13(12), 1-9. https://doi.org/10.1007/s12517-020-05450-3
[6] Van Paassen, L. A. (2009). Biogrout, ground improvement by microbial induced carbonate precipitation. [Doctoral, Delft University of Technology]. Netherlands. https://repository. tudelft.nl/islandora/object/uuid:5f3384c4-33bd-4f2a-8641-7c665433b57b?collection=res earch
[7] Kano, S., Moriwaki, T., & Ochi, K. (2017). A Study on the Bio-treatment Technique of Ground Improvement with Urease Microorganisms Which Live in Japan. In Geotechnical Hazards from Large Earthquakes and Heavy Rainfalls. Springer https://doi.org/10.1007/978-4-431-56205-4_41
[8] Akyol, E., Bozkaya, Ö., & Dogan, N. M. (2017). Strengthening sandy soils by microbial methods. Arabian Journal of Geosciences, 10(15), 327. https://doi.org/10.1007/s1 2517-017-3123-9
[9] Jiang, N-J., & Soga, K. (2017). The applicability of microbially induced calcite precipitation (MICP) for internal erosion control in gravel–sand mixtures. Géotechnique, 67(1), 42-55. https://doi.org/10.1680/jgeot.15.P.182
[10] Kucharski, E., Cord-ruwisch, R., Whiffin, V., & Al-thawadi, S. M. J (2006). Microbial Biocementation. (WO/2006/066326). IP Australia. https://patentscope.wipo.int/sea rch/en/detail. jsf?docId=WO2006066326
[11] Lowenstam, H. A., & Weiner, S. (1989). On biomineralization. Oxford University Press. https://www.amazon.com/Biomineralization-Heinz-Lowenstam/dp/0195049 772
[12] Sharma, S. S., & Fahey, M. (2003). Degradation of Stiffness of Cemented Calcareous Soil in Cyclic Triaxial Tests. Journal of Geotechnical and Geoenvironmental Engineering, 129(7), 619-629. https://doi.org/10.1061/(ASCE)1090-0241(2003)129:7(619)
[13] Pourbabaee, A. A., Malekzadeh, F., Sarbolouki, M. N., & Mohajeri, A. (2005). Decolorization of Methyl Orange (As a Model Azo Dye) by the Newly Discovered Bacillus Sp. Iranian Journal of Chemistry and Chemical Engineering 24(3), 41-45. https://doi.org/10.30492/ijcc e.2005.8114
[14] Mata, J. A., Martínez-Cánovas, J., Quesada, E., & Béjar, V. (2002). A Detailed Phenotypic Characterisation of the Type Strains of Halomonas Species. Systematic and Applied Microbiology, 25(3), 360-375. https://doi.org/10.1078/0723-2020-00122
[15] Amouzgar, M., Sooudi, M., & Malekzadeh, F. (2008). A highly resistant to toxic oxyanions, halomonas sp. Strain mam. Journal Of Science (University Of Tehran), 33(4), 5-12. http s://www.sid.ir/en/Journal/ViewPaper.aspx?ID=113241
[16] Ajal Loueian, R., & Pakzad, H.R. (2001). Mechanism Of Aeolian Sands Movement In East Of Isfahan Area. Research Journal Of University Of Isfahan "Science", 15(1-2), 53-70. https://www.sid.ir/en/Journal/ViewPaper.aspx?ID=22404
[17] American Society for Testing and Materials (2011). Standard Practice for Classification of Soils for Engineering Purposes (Unified Soil Classification System) (D 2487 – 11). American Society for Testing and Materials. https://www.scirp.org/(S(i43dyn45teexjx455qlt3d2q))/ reference/ReferencesPapers.aspx?ReferenceID=2064058
[18] Burbank, M., Weaver, T., Lewis, R., Williams, T., Williams, B., & Crawford, R. (2013). Geotechnical tests of sands following bioinduced calcite precipitation catalyzed by indigenous bacteria. Journal of Geotechnical and Geoenvironmental Engineering, 139(6), 928-936. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000781
[19] Chou, C-W., Seagren, E. A., Aydilek, A. H., & Lai, M. (2011). Biocalcification of sand through ureolysis. Journal of Geotechnical and Geoenvironmental Engineering, 137(12), 1179-1189. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000532
[20] American Society for Testing and Materials. (2021). Standard Test Methods for a laboratory Compaction Characteristics of Soil Using Standard Effort (12,400 ft-lbf/ft3 (600 kN-m/m3)) ASTM (2021 D 698-78). American Society for Testing and Materials. https://www.astm .org/Standards/D698
[21] Park, B., & Son, Y. (2017). Ultrasonic and mechanical soil washing processes for the removal of heavy metals from soils. Ultrasonics Sonochemistry, 35, 640-645. https ://doi.org/10.1016/j.ultsonch.2016.02.002
[22] Szabo, T. L., & Wu, J. (2000). A model for longitudinal and shear wave propagation in viscoelastic media. The Journal of the Acoustical Society of America, 107(5), 2437-2446. https://doi.org/10.1121/1.428630
[23] Whitaker, J. M., Vanapalli, S., & Fortin, D. (2018). Improving the strength of sandy soils via ureolytic CaCO3 solidification by Sporosarcina ureae. Biogeosciences, 15(14), 4367-4380. https://doi.org/10.5194/bg-15-4367-2018
[24] Ivanov, V., & Chu, J. (2008). Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ. Reviews in Environmental Science and Bio/Technology, 7(2), 139-153. https://doi.org/10.1007/s11157-007-9126-3
[25] Nayanthara, P. G. N., Dassanayake, A. B. N., Nakashima, K., & Kawasaki, S. (2019). Microbial Induced Carbonate Precipitation Using a Native Inland Bacterium for Beach Sand Stabilization in Nearshore Areas. Applied Sciences, 9(15), 1-24. https://doi.org/10.3 390/app9153201
[26] Zhao, Y., Yao, J., Yuan, Z., Wang, T., Zhang, Y., & Wang, F. (2017). Bioremediation of Cd by strain GZ-22 isolated from mine soil based on biosorption and microbially induced carbonate precipitation. Environmental Science and Pollution Research international, 24(1), 372-380. https://doi.org/10.1007/s11356-016-7810-y
[27] Mohammadi, M. (2020). Investigating the effect of variation of Geology Strength Index (GSI) on the geomechanical parameters of rock mass. Karafan Quarterly Scientific Journal, 17(1), 165-180. https://doi.org/10.48301/kssa.2020.112763
[28] Salimbahrami, S. R. (2021). Prediction of compressive strength of concrete with rubber fibers using artificial neural networks. Karafan Quarterly Scientific Journal, 18(1), 81-98. https ://doi.org/10.48301/kssa.2021.131038