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فصلنامه علمی کارافن

استخراج و پیش تغلیظ داروی دوکسوروبیسین با روش استخراج فاز جامد و اندازه گیری آن توسط طیف سنجی مرئی- فرابنفش

نوع مقاله : مقاله پژوهشی (کاربردی)

نویسندگان
1 دانشیار، گروه شیمی، دانشگاه حکیم سبزواری، سبزوار، ایران.
2 استادیار ،گروه شیمی ، دانشگاه فنی و حرفه‌ای، تهران، ایران.
3 دانشجوی ارشد، گروه شیمی، دانشگاه حکیم سبزواری، سبزوار، ایران.
چکیده
دوکسوروبیسین یک داروی ضد سرطان است که از رشد و پیشرفت سلول‌های سرطانی جلوگیری می‌کند. با توجه به این که در برخی نمونه‌ها مقدار دوکسوروبیسین بسیار کم است، بنابراین انتخاب یک روش پیش تغلیظ بسیار مهم و ضروری است. در این مطالعه، استخراج فاز جامد پراکنده، با استفاده از نانو ذرات اکسید گرافن به عنوان یک روش پیش تغلیظ مورد استفاده قرار گرفته است. پارامترهای مؤثر بر استخراج مانند زمان جذب، زمان واجذب، مقادیر جذب، نوع و حجم حلال آلی (حلال شویش) و pH مورد بررسی و بهینه‌سازی قرار گرفت. پس از بهینه‌سازی شرایط تجربی، غلظت دوکسوروبیسین در طول موج 478.5 نانومتر اندازه‌گیری شد. منحنی کالیبراسیون در محدودۀ غلظت دوکسوروبیسین 0/06 تا 6/5 میلی‌گرم بر لیتر خطی و حد تشخیص 0/018 میلی‌گرم برلیتر (LOD=3Sb/m) و انحراف استاندارد نسبی برای اندازه‌گیری محلول دوکسوروبیسین با ده بار تکرار مقدار 2/8 درصد محاسبه شد. عامل غنی‌سازی و بازیابی روش استخراج به ترتیب 58 و 78 درصد به‌دست می‌آید. روش پیشنهادی برای تعیین دوکسوروبیسین در نمونه‌های واقعی با نتایج تجزیه‌ای رضایت بخش همراه بود.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Extraction and Preconcentration of Doxorubicin by Solid Phase Extraction and its Determination by Uv-vis Spectrophotometry

نویسندگان English

Saeid Nazari 1
َAbolfazl Darroudi 2
Shirin Delavar Devin 3
1 Associate Professor, Faculty of Sciences, Department of Chemistry, Hakim Sabzevari University, Sabzevar, Iran.
2 Assistant Professor, Department of Chemistry, Technical and Vocational University, Tehran, Iran.
3 MSc Student, Faculty of Sciences, Department of Chemistry, Hakim Sabzevari University, Sabzevar, Iran.
چکیده English

Doxorubicin is an anticancer drug that prevents the growth and progression of cancer cells. Considering that in some samples the amount of doxorubicin is very low, so choosing a preconcentrative method is very important and necessary. In this study, dispersive solid phase extraction, by using graphen oxide nano particles, was used as an efficient preconcentration method.Different affecting parameters, such as adsorption time, desorption time, amounts of adsorber, type and volume of organic solvent (desorbing solvent) and pH were investigated and optimized.After optimization of the experimental conditions, doxorubicin concentration was measured at wavelength of 478.5 nm. The calibration curve was linear in the 0.06 to 6.5 mgL-1 doxorubicin concentration range, and the detection limit (at an S/N ratio of 3) was 0.018 mgL⁻¹. Relative standard deviation (RSD%) for ten replicate measurement of doxorubicin solution was 2.8%.The enrichment factor and recovery of the extraction method were 58 and 78% respectively. The proposed method was applied to the determination of doxorubicin in real samples with satisfactory analytical results.

کلیدواژه‌ها English

ِDoxorubicin
Graphen Oxide Nano Particles
Solid phase Extraction
Preconcentration
Uv-vis Spectrophotometry
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دوره 20، شماره 1 - شماره پیاپی 61
فنی و مهندسی
بهار 1402
صفحه 375-388

  • تاریخ دریافت 03 بهمن 1401
  • تاریخ بازنگری 26 اسفند 1401
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