Extraction and Preconcentration of Doxorubicin by Solid phase and its Determination by Uv-Vis Spectrophotometry

Document Type : Original Article

Authors

1 Associate Professor, Faculty of Sciences, Department of Chemistry, Hakim Sabzevari University, Sabzevar, Iran

2 Department of Chemistry, Technical and Vocational University, Tehran, Iran

3 MSc student, Faculty of Sciences, Department of Chemistry, Hakim Sabzevari University, Sabzevar, Iran

10.48301/kssa.2023.382526.2423

Abstract

Doxorubicin is an anticancer drug that prevents the growth and progression of cancer cells. Considering that in some samples the amount of doxorubicin is very low, so choosing a preconcentrative method is very important and necessary. In this study, dispersive solid phase extraction, by using graphen oxide nano particles, was used as an efficient preconcentration method.Different affecting parameters, such as adsorption time, desorption time, amounts of adsorber, type and volume of organic solvent (desorbing solvent) and pH were investigated and optimized.After optimization of the experimental conditions, doxorubicin concentration was measured at wavelength of 478.5 nm. The calibration curve was linear in the 0.06 to 6.5 mgL-1 doxorubicin concentration range, and the detection limit (at an S/N ratio of 3) was 0.018 mgL⁻¹. Relative standard deviation (RSD%) for ten replicate measurement of doxorubicin solution was 2.8%.The enrichment factor and recovery of the extraction method were 58 and 78% respectively. The proposed method was applied to the determination of doxorubicin in real samples with satisfactory analytical results.

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Karafan Quarterly Scientific Journal
Volume 20, Issue 1 - Serial Number 61
Technical & Engineering
June 2023

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History

  • Receive Date: 23 January 2023
  • Revise Date: 17 March 2023
  • Accept Date: 24 April 2023

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